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KMID : 0359319910310030259
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Korean Journal of Veterinary Research
1991 Volume.31 No. 3 p.259 ~ p.264
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BioElectrophoretic analysis of the major proteins of race horse erythrocyte membrane
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Abstract
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The proteins of the race horse erythrocyte membrane were analysed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate(SDS-PAGE), and their relations to the fast erythrocyte sedimentation rate(ESR) of the race horse were investigated.
The erythrocyte sedimentation rate of race horse were very fast compared with the human one (33 times <90˚-plastic-ESR/30m) and 25 times <90˚micro-ESR/30m) as fast as the human one) are reported previously.
Although the general protein profiles of the race horse erythrocyte membranes were almost similar to that of human, band 3 content was showing higher in race horse (34.7%) than in human (25.3%).
The glycoprotein profiles of the race horse erythrocyte membranes revealed by periodic acid Schiff´s(PAS) stain showed a marked difference from that of human.
The PAS-1(glycophorin) and PAS-2(sialoglycoprotein) present in human erythrocyte membrane were almost absent from the Holstein and race horse erythrocyte membranes, but PAS-2 was more in only race horse from thai. of human. Instead, the bovine erythrocyte membranes showed a strong PAS-B near the origin of the electrophorograms and the race horse erythrocyte membranes showed a strong PAS-negative band near the end of the electrophorograms, which is named as PAS-E in this study.
These results suggest that the fast sedimentation rate of race horse erythrocyte is due in part to the presence of more band 3 protein fraction and PAS-E glycoproteins in the race horse erythrocytes.
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